Polyclonal antibodies against human cell-surface antigen CD34

An efficient and inexpensive laboratory approach for the generation and the purification of polyclonal antibodies to human antigen CD34 was developed. It was shown that cloned refolded and purified from Escherichia coli recombinant extracellular fragment of CD34 antigen retained immunogenic determin...

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Bibliographic Details
Published inTSitologiia i genetika Vol. 45; no. 3; p. 3
Main Authors Nikolaev, Iu S, Gil'chuk, P V, Gorbatiuk, O B, Fliak, A I, Labyntsev, A Iu, Irodov, D M, Kolibo, D V, Kordium, V A
Format Journal Article
LanguageRussian
Published Ukraine 01.05.2011
Subjects
Animals
Antibodies - immunology
Antibodies - isolation & purification
Antigens, CD34 - genetics
Antigens, CD34 - immunology
Blotting, Western
Cell Line, Tumor
Cloning, Molecular
Electrophoresis, Polyacrylamide Gel
Escherichia coli - genetics
Female
Genetic Vectors
Humans
Immunohistochemistry
Mice
Mice, Inbred BALB C
Recombinant Fusion Proteins - immunology
Recombinant Fusion Proteins - isolation & purification
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Summary:An efficient and inexpensive laboratory approach for the generation and the purification of polyclonal antibodies to human antigen CD34 was developed. It was shown that cloned refolded and purified from Escherichia coli recombinant extracellular fragment of CD34 antigen retained immunogenic determinants of cell-surface expressed CD34. Immunization of mice with unglycosylated truncated recombinant protein elicit polyclonal antibodies specific for the native human antigen CD34. The antibodies generated are applicable for phenotyping of CD34+ cells using immunocytochemistry and flow cytometry assays.
ISSN:0564-3783