Effect of regulation of Y-box protein 1 by RNA interference on the doxorubicin induced mdr1 gene expression in K562 cells

• Published in: Zhōnghuá xuèyèxué zázhì Vol. 32; no. 6; p. 383
• Main Authors: Shen, Hui-Ling, Xu, Wen-Lin, Chen, Qiao-Yun, Wang, Fa-Chun, Fei, Xia
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2011
• Subjects: ATP Binding Cassette Transporter, Sub-Family B; ATP-Binding Cassette, Sub-Family B, Member 1 - genetics; Doxorubicin - pharmacology; Drug Resistance, Neoplasm - drug effects; Drug Resistance, Neoplasm - genetics; Gene Expression - drug effects; Gene Silencing; Humans; K562 Cells; Protein Transport; RNA Interference; RNA, Small Interfering; Y-Box-Binding Protein 1 - genetics
• ISSN: 0253-2727

To investigate the effect of YB-1 on the transcription of induced mdr1 gene expression in K562 cells. K562 cells were treated with doxorubicin (DOX) at different concentrations and times. Expression of mdr1 and YB-1 genes was examined by RT-PCR and P-glycoprotein (P-gp) by flow cytometry. Cyto/nuclear protein was extracted for YB-1 detection by Western blotting. The expression of YB-1 gene in K562 cells was inhibited by YB-1 gene specific RNA interference (RNAi), then the expression of mdr1 and P-gp in YB-1 gene silenced cells treated with DOX was detected. The mdr1 gene as well as its corresponding protein P-gp was highly expressed in DOX exposed K562 cells. DOX up-regulated the expression of YB-1 gene, and promoted YB-1 protein nuclear translocation. On YB-1 gene silenced, the expressions of mdr1 gene and P-gp were obviously down-regulated in DOX treated K562 cells. Doxorubicin can induce the expression of mdr1 gene in K562 cells, which may result from the transcription of mdr1 gene by activated YB-1.