The experimental study of suppressing silicosis fibrosis

• Published in: Zhonghua laodong weisheng zhiyebing zazhi Vol. 29; no. 10; p. 740
• Main Authors: Weng, Ze-ping, Zhang, Ji-jun, Liu, Wei-wei, Chen, Juan, Liu, Yi-min, Yu, Wei, Tang, Li-juan, Chen, Jia-yu, Fang, Mao, Zhang, Cheng, Ye, Geng-xin, Chen, Ling-zhen, Zhong, Xue-yun
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2011
• Subjects: Animals; Bone Marrow Cells - cytology; Hepatocyte Growth Factor - genetics; Hepatocyte Growth Factor - metabolism; Male; Mesenchymal Stromal Cells - metabolism; Pulmonary Fibrosis - chemically induced; Pulmonary Fibrosis - prevention & control; Rats; Rats, Wistar; Silicon Dioxide - toxicity; Silicosis - prevention & control; Transfection
• ISSN: 1001-9391

To compare the difference of effects on SiO(2)-induced alveolitis and early fibrosis between bone marrow-derived mesenchymal-like stem cells (BM-MSCs) and BM-MSCs transfected by pcDNA3.1-HGF and to explore the mechanism of this effects. The Primary BM-MSCs from Wistar male young rats were cultured and labeled by 4, 6-diamidino-2-phenylindole (DAPI). Fifty Wistar rats were randomly divided into 3 groups:model group (10 rats),which was administered with SiO(2) by the trache, the next day,injected PBS via the tail vein; BM-MSCs group (20 rats),which was administered with SiO(2) by the trache, the next day,injected with 1 ml suspension of BM-MSCs via the tail vein; pcDNA3.1-HGF plus BM-MSC group (20 rats),which was administered with SiO(2) by the trache, the next day,injected with 1 ml suspension of BM-MSCs transfected by pcDNA3.1-HGF via the tail vein. On the 14th and 28th days after treatment, half of the animals were sacrificed, respectively, and the lungs were harvested for frozen section to observe the cell