Role of ERK in the hormesis induced by cadmium chloride in HEK293 cells

To detect the role of ERK in the hormesis induced by cadmium chloride in HEK293 cells. HEK293 cells were treated with CdCl2 of 0, 0.0005, 0.005, 0.05, 0.5, 5, 50, 500 micromol/L for 12h and 24h. The ERK inhibitors (PD98059, U0126) and c-raf shRNA were added before the cell was treated with CdCl2. Th...

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Bibliographic Details
Published inWei sheng yan jiu Vol. 40; no. 4; p. 517
Main Authors Hao, Changfu, Hao, Weidong
Format Journal Article
LanguageChinese
Published China 01.07.2011
Subjects
Butadienes - pharmacology
Cadmium Chloride - toxicity
Dose-Response Relationship, Drug
Extracellular Signal-Regulated MAP Kinases - antagonists & inhibitors
Flavonoids - pharmacology
HEK293 Cells
Humans
MAP Kinase Signaling System - physiology
Mitogen-Activated Protein Kinase 3 - metabolism
Nitriles - pharmacology
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Summary:To detect the role of ERK in the hormesis induced by cadmium chloride in HEK293 cells. HEK293 cells were treated with CdCl2 of 0, 0.0005, 0.005, 0.05, 0.5, 5, 50, 500 micromol/L for 12h and 24h. The ERK inhibitors (PD98059, U0126) and c-raf shRNA were added before the cell was treated with CdCl2. The proliferation viability was measured by thethiazolyl blue (MTT) and WST-8 assay. The level of the phosphorylated ERK and c-raf was examined with the western blot method. The level of c-fos, c-myc and c-raf mRNA was examined with RT-PCR. CdCl2 stimulated cell proliferation at lower concentrations (0.05 and 0.5 micromol/L) but inhibit it at higher concentrations (50 and 500 micromol/L). The c-fos mRNA increased significantly at low concentrations (0.51 micromol/L) and the c-myc mRNA increased with CdCl2 concentration. Treated with 100 micromol/L ERK1/2 inhibitor and c-raf shRNA, the cell proliferation did not increase significantly at low concentrations (0.05 and 0.51 micromol/L), but still decreased at high concen
ISSN:1000-8020