Effects of wild type PTEN gene on proliferation, apoptosis and the influence on apoptosis key factor Bcl-2 and Caspase family on K562 cells

• Published in: Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 40; no. 4; p. 679
• Main Authors: Cheng, Zhi-Yong, Liang, Wen-Tong, Niu, Zhi-Yun, Xue, Fang, Yao, Li, Pan, Ling
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2009
• Subjects: Adenoviridae - genetics; Adenoviridae - metabolism; Apoptosis; Caspase 3 - genetics; Caspase 3 - metabolism; Caspase 7 - genetics; Caspase 7 - metabolism; Caspase 9 - genetics; Caspase 9 - metabolism; Caspases - genetics; Caspases - metabolism; Cell Proliferation; Humans; K562 Cells; Proto-Oncogene Proteins c-bcl-2 - genetics; Proto-Oncogene Proteins c-bcl-2 - metabolism; PTEN Phosphohydrolase - genetics; Recombinant Proteins - genetics
• ISSN: 1672-173X

To investigate the effect of tumor-suppressing gene,wild type PTEN gene, mediated by adenovirus vector on the cell proliferation, apoptosis and the influence on apoptosis key factor Bcl-2 and Caspase family on human chronic myeloid leukemia (CML) cell line K562 in vitro. The recombinated Ad-PTEN gene containing green fluorescent protein gene (Ad-PTEN-GFP) or the empty vector (Ad-GFP) was transfected into K562 cells. The growth of K562 cells was evaluated by MTT assay; the transfection efficiency of Ad-PTEN-GFP, apoptosis rate and proliferation index (PI) were assessed by flow cytometry (FCM). Morphological characteristics of transfected cells under light and transmission electron microscope were applied to demonstrated the apoptosis; DNA ladder and fluorescent staining were also tested; the PTEN, Bcl-2 mRNA levels were detected by real-time fluorescent relative-quantification reverse transcriptional PCR (FQ-PCR); PTEN and Bcl-2 protein levels were detected by Western Blotting; and Caspase-3/7 and -9 protein a