Morphological changes associated with low-tone hearing loss in guinea pig models of early endolymphatic hydrops

• Published in: Sheng li hsüeh pao Vol. 64; no. 1; p. 48
• Main Authors: Jia, Xian-Hao, Liang, Qin, Chi, Zhang-Cai, Dai, Pei-Dong, Zhang, Tian-Yu, Wang, Tian-Feng
• Format: Journal Article
• Language: Chinese
• Published: China 25.02.2012
• Subjects: Animals; Cochlea - pathology; Cochlea - physiopathology; Endolymphatic Hydrops - complications; Endolymphatic Hydrops - physiopathology; Guinea Pigs; Hair Cells, Auditory, Outer - pathology; Hearing Loss, Sensorineural - etiology; Hearing Loss, Sensorineural - pathology; Hearing Loss, Sensorineural - physiopathology; Male
• ISSN: 0371-0874

The present study was to explore the functional and morphological changes in cochleas of guinea pig models of early endolymphatic hydrops. Thirty albino guinea pigs were randomly divided into three groups: control, 4-week model and 8-week model groups. For each group, n = 10. Model groups were operated on the right ears to result in endolymphatic hydrops with the method of slight destruction of endolymphatic sac and duct from extradural posterior cranial fossa approach, and the animals in control group were sham operated. Electrocochleogram recorded by trans-tympanic approach and auditory brainstem response (ABR) were tested in preoperative model groups, control group, 4-week model group and 8-week model group to assess the hearing changes. Histologic morphometry was used to quantify hydrops by testing scala media area (SMA) ratio. Scanning electron microscope was used to assess the changes of cochlea hair cells. The results showed that the summating potential/compound action potential (SP/AP) ratio of electr