Efficacy of acetated-Ringer containing 10% dextran: evaluation in a rat model of acute normovolemic hemodilution

• Published in: Masui. The Japanese journal of anesthesiology Vol. 59; no. 2; p. 164
• Main Authors: Kitamura, Takayuki, Iwanaga, Shoko, Ohno, Nagara, Sato, Kanako, Otsuji, Mikiya, Ogawa, Makoto, Kojima, Jun, Yamada, Yoshitsugu
• Format: Journal Article
• Language: Japanese
• Published: Japan 01.02.2010
• Subjects: Animals; Blood Volume; Dextrans; Hemodilution; Isotonic Solutions; Lactic Acid - blood; Male; Oxygen Consumption; Rats; Rats, Sprague-Dawley
• ISSN: 0021-4892

In this study, we prepared acetated-Ringer containing 10% dextran, and evaluated its efficacy in a rat model of acute normovolemic hemodilution (ANH). Under sevoflurane anesthesia, 21 rats (350-400 g) underwent ANH. In all rats 10 ml of blood was withdrawn, and they were infused with 10 ml of acetated-Ringer containing 10% dextran (Group-A, n = 11) or lactated-Ringer containing 10% dextran (Group-L, n = 10). For each rat, ANH procedure took 20 minutes. Hemodynamics and arterial blood gas data before and after ANH were compared. All results but for blood lactate level after ANH were comparable between the 2 groups. Blood lactate level was elevated in both groups after ANH; however, the elevation was significantly higher in Group-L. No differences in hemodynamics after ANH reveal that 2 plasma substitutes used in this study have almost the same quality in maintaining intravascular volume. Blood lactate level is generally recognized as an indicator of tissue hypoxia. A possible explanation for the significantly higher blood lactate level in Group-L after ANH is that exogenous overload of lactate as well as tissue hypoxia might have elevated blood lactate levels after ANH in this study. These results suggest the efficacy of acetated-Ringer containing 10% dextran.