The expression of Cyclin A and p21cip1 in fibroblast of hypertrophic scar

• Published in: Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery Vol. 26; no. 4; p. 295
• Main Authors: Jin, Wen-Hu, Wang, Da-Li, Tang, Hong-Mei, Zou, An-Fang, Shao, Wei
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2010
• Subjects: Adolescent; Adult; Cell Cycle; Cells, Cultured; Child; Cicatrix, Hypertrophic - metabolism; Cicatrix, Hypertrophic - pathology; Cyclin A - metabolism; Cyclin-Dependent Kinase Inhibitor p21 - metabolism; Female; Fibroblasts - metabolism; Fibroblasts - pathology; Humans; Male; Middle Aged; Young Adult
• ISSN: 1009-4598

To study the relation of the mRNA and protein expression of Cyclin A and p21cip1 in different stages hypertrophic scar fibroblast (FB) with its cell cycle, so as to provide theoretical evidence for intervention therapy of cell cycle gene being used in hypertrophic scar. The hypertrophic scar samples at different stages (the third month, the sixth month, the twelfth month, the twenty-fourth month) in 32 cases and the normal skin samples in 8 cases were used in this study. The expression of Cyclin A, p21cip1 mRNA and protein was detected by quantitative real-time PCR and Western Blot. And at the same time, the flow cytometer was used to detect the fibroblastic cell life cycle. 1) The expression of Cyclin A mRNA and protein in the third month group, the sixth month group, the twelfth month group, the twenty-fourth month group were 19.34 +/- 2.41, 0.99 +/- 0.11; 19.30 +/- 1.42, 0.96 +/- 0.09; 10.73 +/- 2.93, 0.66 +/- 0.58; 9.29 +/- 0.97, 0.65 +/- 0.14, respectively. And in corresponding stages, the expression of