Synergetic transactivating functions of HCV core and truncated HBV middle surface protein

• Published in: Zhonghua gan zang bing za zhi Vol. 10; no. 5; p. 354
• Main Authors: Liu, Yan, Cheng, Jun, Wang, Gang, Li, Ke, Duan, Huijuan, Wang, Lin, Li, Li, Zhang, Ling Xia, Chen, Jumei
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2002
• Subjects: beta-Galactosidase; Hep G2 Cells; Hepatitis B - genetics; Hepatitis B - metabolism; Hepatitis C - genetics; Hepatitis C - metabolism; Humans; Membrane Proteins - genetics; Membrane Proteins - metabolism; Plasmids; Promoter Regions, Genetic; Transcriptional Activation; Transfection; Viral Core Proteins - genetics; Viral Core Proteins - metabolism
• ISSN: 1007-3418

To investigate the synergetic transactivating functions of HCV core and truncated HBV middle surface proteins. Two recombinant expression plasmids harboring HCV core and C-terminally truncated HBV middle surface protein gene were constructed, respectively. The plasmids were transfected into HepG2 cells and cotransfected HepG2 cells with reporter plasmid pSV-lacZ by lipofectamine plus reagents. The transient expressed viral proteins were identified at the transcription and translation levels. The activity of beta-galactosidase was detected, which reflected the transactivating function of the proteins. The protein expression of plasmids was detected in soluble cell extracts of transiently transfected HepG2 cells. HCV core protein activated the beta-galactosidase expression at a value of 4.6 times higher than the control, while C-terminally truncated HBV middle surface protein activated at a value of 3.2 times. It reached 8.4 times transfected with the plasmids simultaneously. The transactivating effect was dose