Effect of triptolide on airway remodeling and the expression of phosphoinositide 3-kinases in asthmatic rats

To explore the effect of Triptolide on airway remodeling and the expression of Phosphoinositide 3-Kinases in asthmatic rats. 40 rats were randomly divided into 5 groups (n = 8): (1) Control group; (2) Asthmatic 4 weeks group; (3) Asthmatic 6 weeks group; (4) Therapeutic 4 weeks group; (5) Therapeuti...

Full description

Saved in:
Bibliographic Details
Published inZhongguo ying yong sheng li xue za zhi Vol. 23; no. 3; p. 359
Main Authors Mo, Bi-Wen, Wang, Chang-Ming, Zhang, Zhen-Xiang, Xu, Yong-Jian, Xiong, Wei-Ning, Liu, Xian-Sheng, Fang, Chun-Sheng
Format Journal Article
LanguageChinese
Published China 01.08.2007
Subjects
Airway Remodeling
Animals
Asthma - metabolism
Asthma - physiopathology
Diterpenes - pharmacology
Epoxy Compounds - pharmacology
Male
Phenanthrenes - pharmacology
Phosphatidylinositol 3-Kinase - metabolism
Rats
Rats, Sprague-Dawley
Signal Transduction
Online AccessGet more information

Cover

More Information
Summary:To explore the effect of Triptolide on airway remodeling and the expression of Phosphoinositide 3-Kinases in asthmatic rats. 40 rats were randomly divided into 5 groups (n = 8): (1) Control group; (2) Asthmatic 4 weeks group; (3) Asthmatic 6 weeks group; (4) Therapeutic 4 weeks group; (5) Therapeutic 6 weeks group. The airway resistance and eosinophilic inflammation of airway wall were observed. The airway wall thickness (WA/Pi), the bronchial smooth muscle thickness (smooth muscle area/Pi) and the number of bronchial smooth muscle nucleus (N/Pi) were measured by image analysis system. The expression of PI3K protein and mRNA were determined by immunohistochemical staining and reverse transcription-polymerase chain reaction (RT-PCR). (1) The expression of PI3K p85alpha protein and mRNA in asthmatic 4 weeks group and asthmatic 6 weeks group were significantly higher than control group, respectively (P < 0.01). The above-mentioned parameters of therapeutic 6 weeks group were significantly lower than those of ast
ISSN:1000-6834