Prokaryote diversity in water environment of land-ocean ecotone of Zhuhai City

By constructing 16S rDNA clone library with PCR-RFLP, the prokaryote diversity in the seawater and groundwater of land-ocean ecotone of Zhuhai City was investigated, and the similarity and cluster analyses were implemented with the database of the sequences in Genbank. In the seawater, Proteobacteri...

Full description

Saved in:
Bibliographic Details
Published inYing yong sheng tai xue bao Vol. 21; no. 2; p. 452
Main Authors Huang, Xiao-Lan, Chen, Jian-Yao, Zhou, Shi-Ning, Xie, Li-Chun, Fu, Cong-Sheng
Format Journal Article
LanguageChinese
Published China 01.02.2010
Subjects
Archaea - classification
Archaea - genetics
Archaea - isolation & purification
Biodiversity
China
DNA, Archaeal - analysis
DNA, Bacterial - analysis
DNA, Ribosomal - genetics
Environmental Monitoring
Fresh Water
Oceans and Seas
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Proteobacteria - classification
Proteobacteria - genetics
Proteobacteria - isolation & purification
Seawater - microbiology
Water Microbiology
China
Online AccessGet more information

Cover

More Information
Summary:By constructing 16S rDNA clone library with PCR-RFLP, the prokaryote diversity in the seawater and groundwater of land-ocean ecotone of Zhuhai City was investigated, and the similarity and cluster analyses were implemented with the database of the sequences in Genbank. In the seawater, Proteobacteria was dominant, followed by Archaeon, Gemmatimonadetes, Candidate division OP3 and OP8, and Planctomycetes, etc.; while in the groundwater, Archaeon was dominant, followed by Proteobacteria, Sphingobacteria, Candidate division OP3, Actinobacterium, and Pseudomonas. The dominant taxa in the groundwater had high similarity to the unculturable groups of marine microorganisms. Large amount of bacteria capable of degrading organic matter and purifying water body existed in the groundwater, suggesting that after long-term evolution, the land-ocean ecotone of Zhuhai City had the characteristics of both land and ocean.
ISSN:1001-9332