Associated factors for the infection of Beijing genotype Mycobacterium tuberculosis

• Published in: Zhonghua jiehe he huxi zazhi Vol. 33; no. 2; p. 132
• Main Authors: Fan, Lin, Gui, Xu-Wei, Qin, Lian-Hua, Wang, Jie, Hu, Zhong-Yi, Tang, Sheng-Jie, Xiao, He-Ping
• Format: Journal Article
• Language: Chinese
• Published: China 01.02.2010
• Subjects: China - epidemiology; DNA, Bacterial - genetics; Female; Genotype; Humans; Male; Middle Aged; Mycobacterium tuberculosis - genetics; Mycobacterium tuberculosis - isolation & purification; Prevalence; Risk Factors; Sputum - microbiology; Tuberculosis, Multidrug-Resistant - genetics; Tuberculosis, Pulmonary - epidemiology; Tuberculosis, Pulmonary - genetics; Tuberculosis, Pulmonary - microbiology; China
• ISSN: 1001-0939

to analyze the risk factors for the infection of Beijing genotype Mycobacterium tuberculosis (MTB) and the relationship to drug resistance and clinical symptoms. sputum samples were collected from patients with pulmonary tuberculosis who were admitted to the hospital during July, 2007 to March, 2008. The sputum was cultured with L-J method, and then the bacterial DNA was isolated and genotyped with VNTR-7 (variable-number tandem repeats, VNTR) and RD105 deletion method respectively. The association between different genotypes and risk factors was analyzed. a hundred and sixteen clinical sputum isolates were obtained from 172 positive sputum cases. There were 112 isolates of MTB, and isolates of non-tuberculosis mycobacterium (NTM). Among the 97 isolates from Shanghai, Zhejiang and Jiangsu areas, Beijing genotype accounted for 86.6% (84/97), and non-Beijing genotype for 13.4% (13/97). The rates of MDR (multi-drug resistance), PDR (poly-drug resistance) and single drug resistance in Beijing genotype were not si