A pilot investigation on serum protein fingerprinting of nasopharyngeal carcinoma

• Published in: Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery Vol. 46; no. 6; p. 509
• Main Authors: Huang, Xiao-wen, Zhou, Wei, Chen, Jin, Chu, Han-qi, Xiao, Cai-wen, Hua, Xiao-yang, Zhang, Ping, Li, Hui-qin, Cui, Yong-hua
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2011
• Subjects: Adult; Aged; Biomarkers, Tumor - blood; Blood Proteins - analysis; Carcinoma; Carcinoma, Squamous Cell - blood; Carcinoma, Squamous Cell - pathology; Case-Control Studies; Female; Humans; Male; Mass Spectrometry; Middle Aged; Nasopharyngeal Carcinoma; Nasopharyngeal Neoplasms - blood; Nasopharyngeal Neoplasms - pathology; Neoplasm Staging; Peptide Mapping; Pilot Projects; Proteomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• ISSN: 1673-0860

To establish a preliminary foundation for developing a serum proteomics diagnostic model of nasopharyngeal carcinoma (NPC) by comparing the differences in serum protein fingerprints between patients with NPC and healthy subjects and between different types of NPC. The serum samples of 41 patients with different types of NPC and 20 healthy subjects were collected. Surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS) were used to detect the blood samples to obtain serum protein mass spectrum, i.e. serum protein fingerprinting. Biomarker Wizard and Biomarker Patterns system software were used to compare the differences in serum protein mass spectrum between NPC patients and healthy subjects and between different types of NPC, and to screen out the NPC-related serum proteins. Compared with the healthy control, NPC patients emerged 9 very prominent protein peaks (P < 0.001), with the combined differential peaks. No significant difference was found in relative amount of serum