Increased testicular 8-hydroxy-2'-deoxyguanosine (8-OHdG) and inducible nitric oxide synthetase (iNOS) and nuclear factor κB (NF-κB) expressions in experimental rat varicocele

To assess nuclear factor-kappaB (NF-kappaB), inducible NO synthase (iNOS) immunohistochemically, and 8-hydroxy-2'-deoxyguanosine (8-OHdG) biochemically, which are sensitive biological markers of oxidative damage and stress, in testes with experimental varicocele. Adult rats were randomly divide...

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Published inArchivio italiano di urologia, andrologia Vol. 82; no. 4; p. 148
Main Authors Tuğcu, Volkan, Gedikbaşi, Asuman, Mutlu, Bircan, Güner, Ekrem, Uhri, Mehmet, Andican, Gülnur, Ozbek, Emin, Taşçi, Ali I
Format Journal Article
LanguageEnglish
Published Italy 01.12.2010
Subjects
Animals
Deoxyguanosine - analogs & derivatives
Deoxyguanosine - biosynthesis
Male
NF-kappa B - biosynthesis
Nitric Oxide Synthase Type II - biosynthesis
Rats
Rats, Sprague-Dawley
Testis - enzymology
Testis - metabolism
Up-Regulation - physiology
Varicocele - enzymology
Varicocele - metabolism
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Summary:To assess nuclear factor-kappaB (NF-kappaB), inducible NO synthase (iNOS) immunohistochemically, and 8-hydroxy-2'-deoxyguanosine (8-OHdG) biochemically, which are sensitive biological markers of oxidative damage and stress, in testes with experimental varicocele. Adult rats were randomly divided into three groups. Control group (n: 10), sham group (n: 10), varicocele group (n: 10). Of 14 rats undergoing partial ligation of the left renal vein, 10 rats had developed dilation of the left spermatic vein when evaluated 3 months after varicocele-inducing surgery. The rats were sacrificed after 3 months of the varicocele-inducing surgery. Ipsilateral and contralateral testes were examined for 8-hydroxy-2'-deoxyguanosine (8-OHdG) biochemically, inducible NO synthase (iNOS) and nuclear factor-kappaB (NF-kappaB) expression immunohistochemically. Inducible NO synthase (iNOS), nuclear factor-kappaB (NF-kappaB) expressions and 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels in both testes of varicocele group were markedly higher compared with control and sham groups (p < 0.01). There was no difference between control and sham groups (p > 0.05). Regarding to our results, we suggest that varicocele may produce oxidative stress in both of testes, and we believe that this stress may play a role in male fertility.
ISSN:1124-3562