Effect of naloxone on expression of Bcl-2 protein and tumor necrosis factor-alpha in rats with acute myocardial ischemia/reperfusion injury

• Published in: Zhongguo wei zhong bing ji jiu yi xue Vol. 17; no. 7; p. 430
• Main Authors: Tang, Xiao-qin, Zhao, Jian-hong, Zhang, Zheng-yi, Zhang, Xu, Song, Shao-li
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2005
• Subjects: Animals; Apoptosis; Disease Models, Animal; Myocardial Reperfusion Injury - metabolism; Myocardial Reperfusion Injury - pathology; Myocardium - metabolism; Myocardium - pathology; Naloxone - pharmacology; Proto-Oncogene Proteins c-bcl-2 - metabolism; Random Allocation; Rats; Rats, Sprague-Dawley; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 1003-0603

To investigate the effect of naloxone on myocardial cell apoptosis and apoptosis-related gene Bcl-2 in rats with acute myocardial ischemia/reperfusion (AMIR) injury, and explore the mechanism of protective effect of naloxone on myocardium. Thirty SD rats were randomly divided into three groups (n=10): ischemia/reperfusion group, naloxone preconditioning group (naloxone was injected intraperitoneally 10 minutes before ischemia and 2 hours after reperfusion), and normal control group. The left anterior descending branch (LAD) of rat coronary artery was tied and un-tied in ischemia/reperfusion group and naloxone preconditioning group to establish the AMIR model in rats. The animals were then sacrificed and hearts were harvested. The expression of Bcl-2 protein was observed by immunohistochemical technique. Radioimmunoassay (RIA) was used to determine tumor necrosis factor-alpha (TNF-alpha) in serum. In the normal control group, there was no Bcl-2 expression and TNF-alpha level was (0.39+/-0.06) mug/L. Higher exp