Effects of FMS-like tyrosine kinase 3 targeted RNA interference on proliferation and apoptosis of acute monocytic leukemia cell line THP-1

• Published in: Zhonghua er ke za zhi Vol. 45; no. 8; p. 615
• Main Authors: Lu, Jie, Sheng, Guang-yao, Zou, Xiang, Xu, Xue-ju, Zhao, Xiao-ming, Bai, Song-ting, Xu, Pei-rong
• Format: Journal Article
• Language: Chinese
• Published: China 01.08.2007
• Subjects: Apoptosis - drug effects; Apoptosis - genetics; Cell Proliferation - drug effects; Child; fms-Like Tyrosine Kinase 3 - metabolism; Humans; Leukemia, Monocytic, Acute - enzymology; Leukemia, Monocytic, Acute - pathology; Protein-Tyrosine Kinases - metabolism; Receptor Protein-Tyrosine Kinases - metabolism; RNA Interference - physiology; RNA, Small Interfering - pharmacology
• ISSN: 0578-1310

FMS-like tyrosine kinase 3 (FLT3) is a receptor tyrosine kinase that is constitutively activated in (70-90)% pediatric patients with acute myeloid leukemia (AML) and appears to confer an adverse prognosis. Although several FLT3-selective small molecule inhibitors and antibodies were developed with varied degrees of success, to address the specificity and resistance, new approaches for specifically targeted FLT3 are needed and RNA interference is a promising choice. The aim of the present study was to investigate the efficacy of suppression of FLT3 induced by small hairpin interfering RNA (shRNA) on myeloproliferation and apoptosis in an acute monocytic leukemia (AMOL) cell line THP-1. FLT3-targeted small hairpin interfering RNA (FLT3-shRNA) was designed and synthesized by transcription system in vitro was transfected into THP-1 cells. Firstly FLT3 mRNA level was detected by semi-quantitative RT-PCR and FLT3 protein level was detected by flow cytometry (FCM) to verify the efficacy on FLT3-shRNA interference at