Effect of reperfusion leukocyte-depleted blood on canine myocardial energy metabolism balance during cardiopulmonary bypass

• Published in: Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 38; no. 4; p. 671
• Main Authors: Fang, Hua, Li, Chang-xi, Wang, Quan-yun, Liu, Jin
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2007
• Subjects: Adenosine Triphosphate - metabolism; Animals; Cardiopulmonary Bypass; Dogs; Energy Metabolism; Female; Glutathione Peroxidase - metabolism; Leukocytes - metabolism; Male; Malondialdehyde - metabolism; Mitochondrial Swelling; Myocardium - metabolism; Myocardium - pathology; Peroxidase - metabolism; Reperfusion; Superoxide Dismutase - metabolism
• ISSN: 1672-173X

To investigate the impact of myocardial energy metabolism of canine reperfused with leukocyte-depleted blood during cardiopulmonary bypass (CPB). Eighteen adult healthy dogs undergoing CPB were randomly divided into 3 groups: the control group (group C, n=6), whole blood (group W, n=6) and the experimental group (group L, n=6) with use of the leukocyte depletion filter (LDF) on the bypass circuit. The contents of adenine nucleotide, superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), myeloperoxidase (MPO), malondialdehyde (MDA) and mitochondrial swelling of myocardia were determined respectively before cross-clamping, at 40 min and 60 min after aortic cross-clamping (AC), 30 min and 60 min after declamping (DC) during CPB. Reperfused with leukocyte-depleted blood by LDF connected with bypass circuit, the dog hearts of group L at 60 min after AC, 30 min and 60 min after DC were much better in the recovery of myocardium energy metabolism, higher in contents of myocardium SOD and GSH-PX than those in gr