The effect of allogenic hematopoietic stem cell transplantation on tumor recurrence and metastasis of hepatocellular carcinoma after hepatectomy and the relationship with presence of AFP mRNA and VEGF-C mRNA in peripheral blood

To evaluate the effect of alloHST on recurrence and metastasis of HCC after hepatic radical resection and investigate the relationship between AFP mRNA, VEGF-C mRNA and recurrence and metastasis of HCC after hepatic radical resection. 22 SCID mice were randomized into 3 groups: group A-the scheduled...

Full description

Saved in:
Bibliographic Details
Published inSichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 41; no. 2; p. 256
Main Authors Deng, Zhi-gang, Li, Bo, Zu, Cun
Format Journal Article
LanguageChinese
Published China 01.03.2010
Subjects
alpha-Fetoproteins - genetics
alpha-Fetoproteins - metabolism
Animals
Carcinoma, Hepatocellular - pathology
Carcinoma, Hepatocellular - surgery
Carcinoma, Hepatocellular - therapy
Hematopoietic Stem Cell Transplantation
Hepatectomy
Liver Neoplasms - pathology
Liver Neoplasms - surgery
Liver Neoplasms - therapy
Liver Transplantation
Male
Mice
Mice, Nude
Mice, SCID
Neoplasm Metastasis - prevention & control
Neoplasm Recurrence, Local - prevention & control
Postoperative Period
Random Allocation
RNA, Messenger - genetics
RNA, Messenger - metabolism
Vascular Endothelial Growth Factor C - genetics
Vascular Endothelial Growth Factor C - metabolism
Online AccessGet more information

Cover

More Information
Summary:To evaluate the effect of alloHST on recurrence and metastasis of HCC after hepatic radical resection and investigate the relationship between AFP mRNA, VEGF-C mRNA and recurrence and metastasis of HCC after hepatic radical resection. 22 SCID mice were randomized into 3 groups: group A-the scheduled transplantation, group, B-the single transplantation, and group C-the normal saline group as control. Human umbilical cord blood was transplanted into SCID mice by tail vein, Six weeks after AlloHST, the orthotopic tumor model in SCID mice was established by implanting histologically intact tissue under the embrane of liver. Ten days later, the mice received resection of lobe bearing tumor. The condition of recurrence and metastasis was observed 4 weeks after operation. All groups were compared by routine pathological test and the expression of AFP mRNA and MAGE-1 mRNA in peripheral blood were examined by real time quantitative reverse transcription-polymerase chain reaction (RQ-PCR). All of the incidence of intra
ISSN:1672-173X