Rhein lysinate induces apoptosis in breast cancer SK-Br-3 cells by inhibiting HER-2 signal pathway

• Published in: Yao hsüeh hsüeh pao Vol. 43; no. 11; p. 1099
• Main Authors: Lin, Ya-Jun, Huang, Yun-Hong, Zhen, Yong-Zhan, Liu, Xiu-Jun, Zhen, Yong-Su
• Format: Journal Article
• Language: Chinese
• Published: China 01.11.2008
• Subjects: Anthraquinones - pharmacology; Antineoplastic Agents - pharmacology; Apoptosis - drug effects; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Cell Line, Tumor; Cell Proliferation - drug effects; Cyclin-Dependent Kinase Inhibitor p21 - metabolism; Female; Humans; Inhibitory Concentration 50; Lysine - pharmacology; NF-kappa B - metabolism; Receptor, ErbB-2 - genetics; Receptor, ErbB-2 - metabolism; RNA, Messenger - metabolism; Signal Transduction; Tumor Suppressor Protein p53 - metabolism
• ISSN: 0513-4870

This study is to investigate the effect of rhein lysinate on inducing human breast cancer cell line SK-Br-3 apoptosis and the role of HER-2 signal pathway in the apoptosis. MTT assay was used to detect SK-Br-3 cell proliferation. Cell cycle and apoptosis were analyzed by flow cytometry. The protein expression and the protein phosphorylation of HER-2 signal pathway were detected by Western blotting. The level of HER-2 mRNA was detected by RT-PCR and the level of HER-2 expression was also detected by immunofluorescence cytochemical methods. The results showed that rhein lysinate remarkably inhibited breast cancer SK-Br-3 cell proliferation. The IC50 value for 48 h treatment was 85 micromol x L(-1). Apoptosis in SK-Br-3 cells was induced by rhein lysinate in a dose dependent manner. The protein expressions of HER-2, NF-KB, and the protein phosphorylation of HER-2 were downregulated, however the protein expression of p53 and p21 was upregulated after rhein lysinate treatment. The level of HER-2 mRNA decreased by