Biological characteristics of a chimeric rabies virus expressing canine parvovirus VP2 protein

To obtain a bivalence vaccine against canine rabies virus and canine parvovirus, a chimeric rabies virus expressing canine parvovirus VP2 protein was generated by the technique of reverse genetics. It was shown that the chimeric virus designated as HEP-Flury (VP2) grew well on BHK-21 cells and the V...

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Published inBingdu xuebao Vol. 25; no. 5; p. 362
Main Authors Niu, Xue-Feng, Liu, Xiao-Hui, Sun, Zhao-Jin, Shi, He-He, Chen, Jing, Jiang, Bido, Sun, Jing-Chen, Guo, Xiao-Feng
Format Journal Article
LanguageChinese
Published China 01.09.2009
Subjects
Animals
Capsid Proteins - genetics
Capsid Proteins - immunology
Cell Line
Cricetinae
Female
Mice
Parvovirus, Canine - genetics
Parvovirus, Canine - immunology
Rabies - immunology
Rabies - virology
Rabies virus - genetics
Rabies virus - immunology
Reverse Transcriptase Polymerase Chain Reaction
Viral Vaccines - genetics
Viral Vaccines - immunology
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Summary:To obtain a bivalence vaccine against canine rabies virus and canine parvovirus, a chimeric rabies virus expressing canine parvovirus VP2 protein was generated by the technique of reverse genetics. It was shown that the chimeric virus designated as HEP-Flury (VP2) grew well on BHK-21 cells and the VP2 gene could still be stably expressed after ten passages on BHK-21 cells. Experiments on the mice immunized with the chimeric virus HEP-Flury (VP2) demonstrated that specific antibodies against rabies virus and canine parvovirus were induced in immunized mice after vaccination with the live chimeric virus.
ISSN:1000-8721