Effect of Chinese herbal drugs and tetrandrine on TGF-beta1 signaling pathway

• Published in: Zhonghua laodong weisheng zhiyebing zazhi Vol. 25; no. 6; p. 336
• Main Authors: Zhang, Hua-nan, Xin, Hong-tao, Zhang, Wei-dong, Xu, Shao-hua, Zhang, Yan, Jia, Qing
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2007
• Subjects: Animals; Benzylisoquinolines - pharmacology; Disease Models, Animal; Drugs, Chinese Herbal - pharmacology; Male; Rats; Rats, Wistar; Signal Transduction - drug effects; Silicosis - drug therapy; Silicosis - metabolism; Smad3 Protein - metabolism; Smad7 Protein - metabolism; Transforming Growth Factor beta1 - metabolism
• ISSN: 1001-9391

To investigate the molecule mechanism of the anti-fibrotic effects of Chinese herbal drugs (Qidan granules) in rats. The male rats were randomly divided into four experimental groups: normal group, model group, Qidan group, tetrandrine group. Every group had 10 rats. Normal group were treated with physiologic saline while others were treated with silicon dioxide (50 mg/rat) by intratracheal instillation to induce silicosis. On 30th day Qidan group and Tetrandrine group were treated with Qidan granules (3125 mg/kg) or treated with tetrandrine (22 mg/kg) respectively. All the rats were scarified after 5 months. Lung/body coefficient was measured. Content of hydroxyproline was measured by alkaline hydrolysis. The gene expression of transforming growth factor-beta1 in bronchoalveolar lavage fluid was examined by using enzyme-linked immunosorbent assay (ELISA). The gene expressions of transforming growth factor-beta1, transcription factor Smad 3 and Smad 7 in lung were analyzed by using immunohistochemical techniq