The effect of propofol preconditioning on cytochrome C release from mitochondria after mild hypothermic ischemia/reperfusion in isolated rat hearts

• Published in: Zhongguo ying yong sheng li xue za zhi Vol. 25; no. 3; p. 318
• Main Authors: Wang, Hao-xing, Zhu, Shan-shan, Zeng, Yin-ming
• Format: Journal Article
• Language: Chinese
• Published: China 01.08.2009
• Subjects: Animals; Apoptosis - drug effects; Apoptosis - physiology; Cytochromes c - metabolism; Hypothermia, Induced; In Vitro Techniques; Ischemic Preconditioning - methods; Male; Mitochondria, Heart - metabolism; Myocardium - metabolism; Myocardium - pathology; Propofol - pharmacology; Propofol - therapeutic use; Random Allocation; Rats; Rats, Sprague-Dawley; Reperfusion Injury - prevention & control
• ISSN: 1000-6834

To explore the effect of propofol preconditioning on cardiomyocyte apoptosis and cytochrome C release from mitochondria during mild hypothermic ischemia/reperfusion in isolated rat hearts. 50 isolated SD rat hearts perfused on Langendorff apparatus were randomly divided into 5 groups (n=10): control group (C), DMSO group (D), 3 different concentrations of propofol groups of 25 micromol x L(-1) (P1), 50 micromol x L(-1) (P2), 100 micromol x L(-1) (P3) propofol respectively. All of the isolated rat hearts were subjected to 31 degrees C mild hypothermic ischemia for 55 min followed by 60 min reperfusion. The D, P1, P2, P3 groups were preconditioned by perfusing with K-H solution containing 20 micromol x L(-1) DMSO and 25, 50, 100 micromol x L(-1) propofol respectively for 10 min and then followed by 5 min K-H solution washing out before ischemia. The preconditioning procedure was repeated twice. Hemodynamics of the hearts was recorded after equilibration(baseline values) immediately before ischemia, 30 min and 6