A NMR-compatible and reduced gravity simulation based (NRG) bioreactor for on-line monitoring cell culture metabolism

We developed a NMR-compatible microgravity-based bioreactor (NRG[R]) that offers the advantage of an analytical non-invasive approach associated to the effects of an optimized suspension culture. The simulated microgravity conditions reached in the bioreactor are analogous to those of commercial app...

Full description

Saved in:
Bibliographic Details
Published inJournal of gravitational physiology Vol. 11; no. 2; p. P191
Main Authors Bradamante, S, Barenghi, L, Villa, A
Format Journal Article
LanguageEnglish
Published United States 01.07.2004
Subjects
Bioreactors
Cell Culture Techniques - instrumentation
Cell Proliferation
Cells, Cultured
Endothelial Cells - cytology
Endothelial Cells - physiology
Equipment Design
Humans
Magnetic Resonance Spectroscopy - instrumentation
Magnetic Resonance Spectroscopy - methods
Weightlessness Simulation - instrumentation
Online AccessGet more information

Cover

More Information
Summary:We developed a NMR-compatible microgravity-based bioreactor (NRG[R]) that offers the advantage of an analytical non-invasive approach associated to the effects of an optimized suspension culture. The simulated microgravity conditions reached in the bioreactor are analogous to those of commercial apparatus like the Rotating Wall Vessel (RWV) system. The faster proliferation of endothelial cells cultured in the NRG bioreactor (doubling time : 28 +/- 1.7 vs. 43 +/- 5.6 h of the control grown in RWV) are attributed to different oxygenation conditions and medium wash out.
ISSN:1077-9248