Expression of survivin in activated T cells and its significance

• Published in: Xi bao yu fen zi mian yi xue za zhi Vol. 21; no. 2; p. 147
• Main Authors: Chen, Bi-cheng, Tang, Li, Zhang, Xin, Guo, Hui, Chang, Sheng, Du, Dun-feng, Chen, Zhong-hua
• Format: Journal Article
• Language: Chinese
• Published: China 01.03.2005
• Subjects: Animals; Apoptosis - immunology; CD3 Complex - immunology; Cell Cycle - immunology; Female; Gene Expression Regulation - immunology; Immunohistochemistry; Inhibitor of Apoptosis Proteins; Interleukin-2 Receptor alpha Subunit - immunology; Kinetics; Male; Mice; Microtubule-Associated Proteins - genetics; Repressor Proteins; RNA, Messenger - genetics; RNA, Messenger - metabolism; Spleen - cytology; T-Lymphocytes - cytology; T-Lymphocytes - immunology; T-Lymphocytes - metabolism; Time Factors
• ISSN: 1007-8738

To investigate the expression of survivin in activated T cells in vitro and in vivo. Expressions of survivin and proliferating cell nuclear antigen (PCNA) in splenocytes stimulated with ConA or alloantigen were detected by immunohistochemical straining. Flow cytometry was used to analyze CD3, CD25 and survivin expressions and apoptosis of cultured splenocytes. Splenocytes from C57BL/6(H-2b) mice were infused into BALB/c(H-2d) nude mice to observe the survivin expression in vivo after alloantigen stimulation. Expressions of survivin and PCNA were detected in stimulated cells. Survivin+ cells were CD3+ T cells, and lymphoblasts could simultaneously express CD3, CD25 and survivin. However, not all survivin+ cells expressed CD25. Five days after culture, apoptotic rate of ConA-stimulated splenocytes increased markedly (P<0.001). From day 4 to 7 after infusion, the alloantigen-activated T cell infiltrating in livers of nude mice expressed survivin. Survivin can be detected in T cells after in vivo and in vitro sti