Sunflower (Helianthus annuus L.)

Sunflower (Helianthus annuus L.) is considered one of the recalcitrant species in terms of transformation and regeneration. A routine transformation system of this crop requires competent cell cultures for efficient plant regeneration as well as an effective method for gene delivery. A transformatio...

Full description

Saved in:
Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 343; p. 291
Main Authors Lewi, Dalia M, Hopp, H Esteban, Escandón, Alejandro S
Format Journal Article
LanguageEnglish
Published United States 2006
Subjects
Agrobacterium tumefaciens - genetics
Agrobacterium tumefaciens - growth & development
Blotting, Southern - methods
Gene Transfer Techniques
Helianthus - embryology
Helianthus - genetics
Helianthus - microbiology
Plants, Genetically Modified - embryology
Plants, Genetically Modified - genetics
Plants, Genetically Modified - microbiology
Polymerase Chain Reaction - methods
Regeneration - genetics
Seeds - embryology
Seeds - genetics
Seeds - microbiology
Sequence Analysis, DNA - methods
Transformation, Genetic
Online AccessGet more information

Cover

More Information
Summary:Sunflower (Helianthus annuus L.) is considered one of the recalcitrant species in terms of transformation and regeneration. A routine transformation system of this crop requires competent cell cultures for efficient plant regeneration as well as an effective method for gene delivery. A transformation system was developed by an Agrobacterium tumefaciens-mediated method using split mature embryonic axis explants from the Ha89 genotype. Mean transformation efficiency obtained (measured as PCR+ plants/treated explants) varied from 1 to 5.2% depending on the use of the EHA105 or the C58 strain containing a plasmid with a gene of agronomic interest. The system developed has applicability to several Agrobacterium strains and plasmids with both reporter genes or genes of agronomic interest. Plants obtained with this protocol were confirmed by PCR and Southern blot. Stable inheritance of transgenes was successfully followed until generation T4 in several independent lines.
ISSN:1064-3745