Beta cell protective effects of sodium tungstate in streptozotocin-induced diabetic rats: glycemic control, blockage of oxidative stress and beta cell histochemistry

• Published in: Iranian biomedical journal Vol. 12; no. 3; pp. 143 - 152
• Main Authors: Heidari, Zahra, Harati, Mehdi, Mahmoudzadeh-Sagheb, Hamid Reza, Moudi, Bita
• Format: Journal Article
• Language: English
• Published: Iran 01.07.2008
• Subjects: Alanine Transaminase - blood; Animals; Aspartate Aminotransferases - blood; Blood Glucose - drug effects; Body Weight - drug effects; Cytoprotection - drug effects; Diabetes Mellitus, Experimental - pathology; Feeding Behavior - drug effects; Glucokinase - metabolism; Glucose - metabolism; Glycogen - metabolism; Immunohistochemistry; Insulin - blood; Insulin-Secreting Cells - drug effects; Insulin-Secreting Cells - pathology; Liver - drug effects; Liver - enzymology; Male; Oxidative Stress - drug effects; Rats; Rats, Wistar; Thiobarbituric Acid Reactive Substances - metabolism; Tungsten Compounds - pharmacology; Beta cells; Sodium tungstate; Diabetes mellitus
• ISSN: 1028-852X

Diabetes is a major public health problem. The development of new therapies that are able to improve glycemia management and even to cure diabetes is of great interest. In this study, protective effects of sodium tungstate against STZ-induced beta-cell damages were investigated. Sixty rats were divided into six groups: control, diabetic, sodium tungstate treated diabetic rats from one week before STZ injection (TDB), food-restricted diabetic (FRD), tungstate treated control, sodium tungstate treated diabetic rats from one week after STZ administration (TDA). We evaluated serum insulin, glucose and glucose tolerance; liver glycogen content, glucokinase (GK) activity; blood and pancreas antioxidant power, lipid peroxidation; and fuchsin-aldehyde histochemical staining of beta-cells. Blood glucose levels of TDB group were lower than other diabetic groups (P<0.01). Blood insulin levels of all diabetic groups were lower than controls (P<0.01). Glucose intolerance improved in TDB animals. Blood and pancreas antioxidant power, liver glycogen contents and GK activities and granulated beta cells increased in TDB rats in comparison with other diabetic groups (P<0.01). Likewise, lipid peroxidation decreased significantly in TDB rats (P<0.01). Results suggested that sodium tungstate if administrated before STZ injection improves glycemic state by a direct effect on pancreatic beta-cells and preserves them by reducing the activity of these cells at the time of STZ injection, reducing STZ-induced oxidative stress, reducing insulin secretion, or all of the above mentioned.