Anti-glioma activity of treatment by bone marrow stromal cells transfected with HSV-tk in the rat

• Published in: Zhōnghuá zhŏngliú zázhì Vol. 29; no. 3; p. 171
• Main Authors: Li, Chun-hui, Jiao, Bao-hua
• Format: Journal Article
• Language: Chinese
• Published: China 01.03.2007
• Subjects: Animals; Apoptosis; Bone Marrow Cells - cytology; Brain - pathology; Bystander Effect; Cell Line, Tumor; Coculture Techniques; Genetic Therapy - methods; Glioma - pathology; Glioma - therapy; Magnetic Resonance Imaging; Random Allocation; Rats; Rats, Sprague-Dawley; Simplexvirus - enzymology; Stromal Cells - cytology; Stromal Cells - enzymology; Stromal Cells - transplantation; Thymidine Kinase - genetics; Thymidine Kinase - metabolism; Transfection
• ISSN: 0253-3766

To study the anti-glioma activity of treatment by bone marrow stromal cells (BMSCs) transfected with AdCMV-tk containing HSV-tk gene in rats. Primary cultured BMSCs were obtained and transfected with HSV-tk (BMSCs/tk) and were injected into contralateral brain of glioma-bearing rats to observe their tropism for glioma cells. RT-PCR was performed to examine the transduct of tk gene after it was transduced into BMSCs. C6 glioma cells were co-cultured with BMSCs transfected with HSV-tk. MTT test was performed to examine its antitumor activity. BMSCs, after being transfected with HSV-tk, were injected into contralateral brain tissue of glioma-bearing rats to show their in vivo antitumor activity. Dynamic MRI was performed to monitor the development of intracranial glioma. Purified BMSCs were obtained by primary cultured bone marrow cells. After being transfected with HSV-TK, the cells still stably displayed extensive tropism for intracranial glioma and transcripted tk gene. RT-PCR showed that BMSCs/tk were transd