Anticoagulant activity of low-molecular-weight sulfated derivatives of galactomannan from Cyamopsis tetragonoloba (L.) seeds

Galactomannan from seeds of Cyamopsis tetragonoloba (L.) Taub. (guar) was depolymerized using immobilized enzymatic preparation celloviridin. A set of fragments whose molecular weights varied from 12.6 to 245.6 kDa was obtained. Sulfated derivatives of components of all fractions were synthesized, i...

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Published inPrikladnaja biohimija i mikrobiologija Vol. 44; no. 1; p. 111
Main Authors Mestechkina, N M, Shcherbukhin, V D, Bannikova, G E, Varlamov, V P, Drozd, N N, Tolstenkov, A S, Makarov, V A, Tikhonov, V E
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.01.2008
Subjects
Anticoagulants - chemical synthesis
Anticoagulants - isolation & purification
Anticoagulants - metabolism
Anticoagulants - pharmacology
Blood Coagulation - drug effects
Cyamopsis - chemistry
Glycoside Hydrolases - metabolism
Hydrolysis
Mannans - isolation & purification
Mannans - metabolism
Mannans - pharmacology
Molecular Weight
Protamines - metabolism
Seeds - chemistry
Sulfates - chemical synthesis
Sulfates - metabolism
Sulfates - pharmacology
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Summary:Galactomannan from seeds of Cyamopsis tetragonoloba (L.) Taub. (guar) was depolymerized using immobilized enzymatic preparation celloviridin. A set of fragments whose molecular weights varied from 12.6 to 245.6 kDa was obtained. Sulfated derivatives of components of all fractions were synthesized, in which the content of HSO3(-) groups was 48.05% +/- 2.31. All preparations exhibited anticoagulant activity, which was recorded in vitro in two tests--aIIa and aXa. The antithrombin activity (aIIa) was high (up to 65-87 U/mg) and did not depend on the molecular weight of a sulfated derivative; in the second test (aXa), the effect of molecular weight was observed. Biospecific electrophoresis allowed us to detect the ability of galactomannan sulfates to form complexes with protamine sulfate, a classic antidote to heparin.
ISSN:0555-1099