The contribution for the diagnosis of the tumor-like polypoid lesions of the gallbladder by SELDI-TOF-MS

To detect the serum specific proteins in tumor-like polypoid lesions of the gallbladder patients and establish diagnostic model. Surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) technique and WCX Magnetic Beads were used to detect the serum proteomic patte...

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Published inChung-hua wai kʿo tsa chih Vol. 46; no. 7; p. 525
Main Authors Luan, Feng-Ming, Ge, Chun-Lin, Ma, Ning, Li, Yong-Zhe, Hu, Chao-Jun, Li, Ning
Format Journal Article
LanguageChinese
Published China 01.04.2008
Subjects
Adult
Aged
Biomarkers, Tumor - blood
Blood Proteins - analysis
Female
Gallbladder Neoplasms - diagnosis
Humans
Male
Middle Aged
Polyps - diagnosis
Sensitivity and Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Summary:To detect the serum specific proteins in tumor-like polypoid lesions of the gallbladder patients and establish diagnostic model. Surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) technique and WCX Magnetic Beads were used to detect the serum proteomic patterns of 23 patients with tumor-like PLG, 21 patients with non tumor-like PLG and 26 normal persons. Biomarker Wizard and Biomarker Patterns Software were used in combination to analyze the data. Preliminary screening out 22 representative specific proteins for the diagnosis of the tumor-like PLG. Analysis system under the conditions set selected 3 specific proteins to establish diagnostic model for the tumor-like PLG. The sensitivity and specificity of the model for the diagnosis of the tumor-like PLG were 100% and 89.4%, respectively. SELDI-TOF-MS technique can select specific protein of the tumor-like PLG, and establish diagnostic model of the tumor-like PLG.
ISSN:0529-5815