Full-length cDNA cloning and biological function analysis of a novel gene FAMLF related to familial acute myelogenous leukemia

• Published in: Zhong hua yi xue za zhi Vol. 88; no. 38; p. 2667
• Main Authors: Li, Jing-gang, Wang, Shao-yuan, Huang, Yuan-mao, Wang, Cheng-yi
• Format: Journal Article
• Language: Chinese
• Published: China 21.10.2008
• Subjects: Adolescent; Adult; Aged; Child; Cloning, Molecular; DNA, Complementary - genetics; Female; Gene Library; Genetic Predisposition to Disease; Humans; Leukemia, Myeloid, Acute - genetics; Male; Middle Aged; Molecular Sequence Data; Neoplasm Proteins - genetics; Open Reading Frames; Proteins - genetics; Sequence Analysis, DNA
• ISSN: 0376-2491

To clone the full-length cDNA of a novel gene related to familial acute myelogenous leukemia (AML) and to demonstrate its molecular mechanisms on the gene level. Bone marrow specimen was obtained from a patient of familial AML, male, aged 11, and peripheral blood samples were obtained from 23 AML patients outside this family, 9 normal persons in this family, and 23 normal persons outside this family. Based on the EST sequence zywb87 (GenBank accession number: CV973101) from a subtractive cDNA library of differential expressed genes constructed in familial AML, SMART-rapid amplification of cDNA ends (SMART-RACE) was applied to clone the full-length cDNA of the novel gene, and bioinformatics was used to predict its biological function, the expression of the novel gene in AML was detected by One-Step RT-PCR. A full-length cDNA of 2313 bp was obtained from the bone marrow specimen of the familial AML patient with complete open reading frame (ORF) of 249 bp. Localized on 1q31.3 of human chromosome, it coded a 82-a