Studies on the changes of 5-hydroxymethyl furfuraldehyde content in Radix Rehmanniae steamed for different time

To find the changing regularity of 5-Hydroxymethyl furfuraldehyde (5-HMF) content in Radix Rehmanniae steamed for different time. HPLC method was used, with C18 column (6 mm x 200 mm, 5 microm), a mixture of methanol-water (15: 85) as the mobile phase. The flow rate was 1.0 mL x min(-1). The UV dete...

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Bibliographic Details
Published inZhongguo zhongyao zazhi Vol. 30; no. 18; p. 1438
Main Authors Li, Jun, Zhang, Li-Ping, Liu, Wei, Zhang, Zhen-Ling, Liu, Li-Juan
Format Journal Article
LanguageChinese
Published China 01.09.2005
Subjects
Chromatography, High Pressure Liquid
Furaldehyde - analogs & derivatives
Furaldehyde - analysis
Hot Temperature
Plants, Medicinal - chemistry
Quality Control
Rehmannia - chemistry
Rhizome - chemistry
Technology, Pharmaceutical - methods
Time Factors
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Summary:To find the changing regularity of 5-Hydroxymethyl furfuraldehyde (5-HMF) content in Radix Rehmanniae steamed for different time. HPLC method was used, with C18 column (6 mm x 200 mm, 5 microm), a mixture of methanol-water (15: 85) as the mobile phase. The flow rate was 1.0 mL x min(-1). The UV detection was setat 284 nm and the column temperature was set at 24 degrees C. The linear range was from 0.01 to 0.08 microg, r = 0.999 9 (n = 5). The average recovery was 100.2% and RSD was 2.7% (n = 5). The 5-HMF content increases as the processing time prolongs in a certain time rang.
ISSN:1001-5302