The protective effect of mild hyperthermia against UV-mimetic agent 4-nitroquinoline-1-oxide (4NQO) in human colon cells

Hyperthermia-induced cellular response has been widely investigated for understanding cell physiology in stressful conditions as well as for therapeutic application using heat shock. In this study, the protective effect of mild hyperthermia-induced cellular response was investigated in RKO human col...

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Bibliographic Details
Published inResearch communications in molecular pathology and pharmacology Vol. 115-116; p. 123
Main Authors Jung, Hwa Jin, Hwang, Jee Na, Seo, Young R
Format Journal Article
LanguageEnglish
Published United States 2004
Subjects
4-Nitroquinoline-1-oxide - toxicity
Cell Line
Colon - cytology
Colon - metabolism
Colony-Forming Units Assay
Comet Assay
Enzyme-Linked Immunosorbent Assay
Humans
Hyperthermia, Induced
Methyl Methanesulfonate - toxicity
Mitotic Index
Mutagens - toxicity
Protective Agents
Tumor Suppressor Protein p53 - analysis
Tumor Suppressor Protein p53 - biosynthesis
Ultraviolet Rays
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Summary:Hyperthermia-induced cellular response has been widely investigated for understanding cell physiology in stressful conditions as well as for therapeutic application using heat shock. In this study, the protective effect of mild hyperthermia-induced cellular response was investigated in RKO human colon cell lines, which harbor wild-type p53. Our data showed that the accumulation of p53 protein was induced without DNA damage in response to mild hyperthermia. Interestingly, the sub-lethal of heat shock at 40 degrees C for 30 minutes in RKO cells showed the protective effect against UV mimetic agent 4-nitroquinoline-1-oxide (4NQO) with the decrease in mitotic index, although other papers had shown the induction of apoptosis in RKO cells under the higher dose of heat stress. Thus, we suggest that the protective effect of sub-lethal heat shock might be applicable for a novel preventive approach to clinical applications of mild hyperthermia.
ISSN:1078-0297