Immunogenicity of the recombinant Bacillus strains with cloned gene of biosynthesis of protective antigen against Bacillus anthracis

• Published in: Molekulârnaâ genetika, mikrobiologiâ i virusologiâ no. 3; p. 15
• Main Authors: Mikshis, N I, Kudriavtseva, O M, Bolothikova, M F, Shulepov, D V, Novikova, L V, Popov, Iu A, Shchukovskaia, T N, Drozdov, I G, Kutyrev, V V
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 2007
• Subjects: Animals; Anthrax - immunology; Anthrax - prevention & control; Anthrax Vaccines - genetics; Anthrax Vaccines - immunology; Antibodies, Bacterial - immunology; Antigens, Bacterial - genetics; Antigens, Bacterial - immunology; Bacillus anthracis - genetics; Bacillus anthracis - immunology; Bacterial Toxins - genetics; Bacterial Toxins - immunology; Cloning, Molecular; Dose-Response Relationship, Immunologic; Guinea Pigs; Mice; Mice, Inbred BALB C; Plasmids - genetics; Plasmids - immunology; Rabbits; Recombinant Proteins - genetics; Recombinant Proteins - immunology
• ISSN: 0208-0613

Microbe Russian Anti-Plague Research Institute, Saratov A hybrid plasmid pUB110PA-1 demonstrating stable functioning in the cells of Bacillus strains and containing the gene of biosynthesis of Bacillus anthracis protective antigen was constructed. The recombinant strains surpassing the anthrax vaccinal cultures in the secreted synthesis of the protective antigen were obtained and their immunological efficacy was assessed. A single inoculation of Guinea pigs with the dose of 5 x 107 spores of the recombinant strains imparted efficient protection against B. anthracis challenge. Immune responses were characterized by high indices of immunity and titers of antibodies to the protective antigen. In contrast to the anthrax vaccinal preparations, the gene-engineering strains imposed no residual virulence for BALB/n mice and Guinea pigs.