Establishment of serum protein pattern model for screening pancreatic cancers by SELDI-TOF-MS technique

• Published in: Chung-hua wai kʿo tsa chih Vol. 46; no. 12; p. 932
• Main Authors: Ma, Ning, Ge, Chun-lin, Luan, Feng-ming, Hu, Chao-jun, Li, Yong-zhe, Liu, Young-feng
• Format: Journal Article
• Language: Chinese
• Published: China 15.06.2008
• Subjects: Biomarkers, Tumor - blood; Blood Proteins - analysis; Early Detection of Cancer; Humans; Mass Screening; Pancreatic Neoplasms - blood; Pancreatic Neoplasms - diagnosis; Proteomics; Sensitivity and Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• ISSN: 0529-5815

To detect the serum specific proteins in pancreatic cancer patients and establish diagnostic model by surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) technique. Twenty-nine serum samples from patients of pancreatic cancer were collected before surgery and an additional 57 serum samples from age and sex matched individuals without cancer were used as controls, SELDI-TOF-MS technique and WCX magnetic beads were used to detect the protein fingerprint expression of all the serum samples and the resulting profiles between pancreatic cancer patients and controls were analyzed with biomarker wizard system, established the model using biomarker patterns system software. A double-blind test was used to determine the sensitivity and specificity of the classification model. A panel of four biomarkers (relative molecular weight are 5705, 4935, 5318 and 3243 Da) were selected to set up a decision trees as the classification model for screening pancreatic cancer effectively. The