Experimental study of repairing damaged human amniotic epithelial cells with formulated fibrin clot and cell growth factor

• Published in: Chung-hua fu chʿan kʿo tsa chih Vol. 41; no. 1; p. 12
• Main Authors: Qi, Hong-bo, Li, Wei, Bian, Du-hong
• Format: Journal Article
• Language: Chinese
• Published: China 01.01.2006
• Subjects: Amnion - pathology; Cell Movement; Cell Proliferation; Cells, Cultured; Cytokines - administration & dosage; Cytokines - pharmacology; Epidermal Growth Factor - administration & dosage; Epidermal Growth Factor - pharmacology; Epithelial Cells - pathology; Epithelial Cells - ultrastructure; Female; Fetal Membranes, Premature Rupture - pathology; Fetal Membranes, Premature Rupture - therapy; Fibrin; Fibroblast Growth Factor 2 - administration & dosage; Fibroblast Growth Factor 2 - pharmacology; Humans; Pregnancy; Transforming Growth Factor beta - administration & dosage; Transforming Growth Factor beta - pharmacology
• ISSN: 0529-567X

To investigate whether damaged human amniotic epithelial cells (HAEC) could be repaired on the matrix of formulated fibrin clot in vitro and the effects of epidermal growth factor (EGF), basic fibroblast growth factor (bFGF) and transforming growth factor beta(1) (TGF-beta(1)) on the proliferation of HAEC. Ring drill was used to drill the HAEC layer on culture sheets to make quantified models of damaged HAEC, on which the lacks were then covered with fibrin clot. Subsequently, EGF (EGF group), bFGF (bFGF group) and TGF-beta(1) (TGF-beta(1) group) of different concentration were added into the sheets respectively. After the predesigned culturing time, the growing and transiting conditions of HAEC were observed under inverted microscope after Giemsa stain. Also, the proliferating conditions of HAEC were detected by using 5-bromodeoxyuridine (BrdU). In all groups, HAEC could transit toward damaged area on fibrin clot and grow there. Higher transiting speed and larger cell numbers were observed in the EGF and bFG