Effect of Kuijieling decoction on proteins expression of IKK-alpha in colonic mucosa of ulcerative colitis model rats

• Published in: Zhong yao cai Vol. 31; no. 7; p. 1016
• Main Authors: Du, Qun, Li, Hong, Wang, Ru-Jun, Li, Yan-Wu, Wu, Yan-Li, Wang, Jian-Hua
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2008
• Subjects: Animals; Colitis, Ulcerative - chemically induced; Colitis, Ulcerative - metabolism; Colitis, Ulcerative - pathology; Disease Models, Animal; Drug Combinations; Drugs, Chinese Herbal - administration & dosage; Drugs, Chinese Herbal - pharmacology; I-kappa B Kinase - metabolism; Immunohistochemistry; Intestinal Mucosa - drug effects; Intestinal Mucosa - metabolism; Intestinal Mucosa - pathology; Male; Plants, Medicinal - chemistry; Random Allocation; Rats; Rats, Sprague-Dawley; Trinitrobenzenesulfonic Acid
• ISSN: 1001-4454

To explore the changes of protein expression of IKK-alpha as well as the effects of Kuijieling Decoction (KD) on colonic mucosa of ulcerative colitis (UC) model rats. UC model rats were induced by TNBS. The rats were randomly divided into six groups: normal control (NC) group, model control (MC) group, Kuijieling low dose (KLD), middle dose (KMD) group, high dose (KHD) group and SASP group. After 10-days' treatment the rats were killed to get their colonic tissues. The positive rate of IKK-alpha expression was detected by immunohistochemical (IHC). The positive rate of IKK-alpha in MC group was significantly higher than that in NC group (P < 0.01). The positive rate of IKK-alpha in KMD group was significantly lower than that in MC group (P < 0.05). The positive rate of IKK-alpha in KHD and SASP group were significantly lower than that in MC group (P < 0.01). IKK-alpha may be involved in the pathogenesis of UC, and KD can inhibit positive rate of IKK-alpha in colonic mucosa of UC model rats induced by TNBS. Th