Experimental study of T lymphocyte reactivity inhibited by allogeneic bone marrow mesenchymal stem cells

• Published in: Zhōnghuá xuèyèxué zázhì Vol. 26; no. 12; p. 740
• Main Authors: Chen, Jian-lin, Feng, Kai, Guo, Zi-Kuan, U, Ren-na, Xu, Chen, Li, Yu-hang, Liu, Xiao-dan, Mao, Ning, Chen, Hu
• Format: Journal Article
• Language: Chinese
• Published: China 01.12.2005
• Subjects: Bone Marrow Cells - immunology; Cell Communication - immunology; Cell Proliferation; Cells, Cultured; Coculture Techniques; Graft vs Host Disease - immunology; Graft vs Host Disease - prevention & control; Humans; Lymphocyte Activation; Mesenchymal Stromal Cells - immunology; T-Lymphocytes - immunology
• ISSN: 0253-2727

To address the question whether bone marrow mesenchymal stem cells (MSCs) could lower responsiveness of allogeneic T lymphocytes against alloantigens, and explore a feasible strategy for prevention of graft versus host disease (GVHD) occurred in allogeneic bone marrow transplantation. T cells were co-cultured with (60)Co-irradiated bone marrow MSCs from different individuals. The proliferative activity of T cells and their reactivity to allogeneic cells and ConA were evaluated with (3)H-TdR incorporation assay. T cells could not be activated upon primary or even secondary exposure to allogeneic MSCs (compared the CPM value of 27,529 +/- 969 of T cell alone with that of primary and secondary exposures to allogeneic MSCs were 9,126 +/- 654 and 13,260 +/- 874, respectively). When MSCs were induced to express HLA-DR, they still could not elicit T cell activation. The proliferation rate of allogenous T cells exposed to MSCs was dramatically declined when T cells from the same donor's MSCs were used as stimulator (