Evaluation on effects of chromogenic medium in rapid detection of Coliform and Escherichia coli

To evaluate the detective efficacy of Chromogenic Coliform and Escherichia Coli Agar (CCEA). A new chromogenic medium CCEA prepared by Huankai laboratory was used to compare with a classical medium of violet red bile agar (VRBA), and other two Chromogenic media Agar I and Agar II by detecting separa...

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Published inZhōnghuá yùfáng-yīxué zázhì Vol. 41; no. 4; p. 307
Main Authors Lu, Mian-fei, Wu, Qing-ping, Cai, Zhi-he, He, Tian-wen
Format Journal Article
LanguageChinese
Published China 01.07.2007
Subjects
Bacteriological Techniques
Culture Media
Enterobacteriaceae - isolation & purification
Escherichia coli - isolation & purification
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Summary:To evaluate the detective efficacy of Chromogenic Coliform and Escherichia Coli Agar (CCEA). A new chromogenic medium CCEA prepared by Huankai laboratory was used to compare with a classical medium of violet red bile agar (VRBA), and other two Chromogenic media Agar I and Agar II by detecting separately 11 reference strains, thirteen sterile samples with Coliform or E.coli and other four samples, and the accordant rates of detection were observed. CCEA had the good selectivity. To seven kinds of quality strains in the resultant analysis, CCEA with VRBA and Agar I had not shown salience difference (P > 0.05), and CCEA with Agar II had significant difference (P < 0.05). CCEA showed more advantages than the Agar II. To thirteen sterile samples with Coliform or E.coli in resultant analysis, CCEA with Agar I and Agar II had shown no significant difference (P > 0.05), while CCEA with VRBA had significant difference (P < 0.05). CCEA might be more advantageous than the VRBA. In analysis of the four actual samples of
ISSN:0253-9624