Research progress of methods of SSR primers development

SSRs is one of molecular markers technology based on DNA length polymorphism and an efficient tool for population genetic studies and primary genetic linkage maps construction. Because of a special primer marker, It's necessary to know a species DNA sequence in order to design primers for PCR t...

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Bibliographic Details
Published inYíchuán Vol. 26; no. 5; p. 769
Main Authors Li, Ming-Fang, Zheng, Xue-Qin
Format Journal Article
LanguageChinese
Published China 01.09.2004
Subjects
Animals
DNA - genetics
DNA Primers
Expressed Sequence Tags
Genomic Library
Humans
Microsatellite Repeats - genetics
Polymorphism, Genetic
Sequence Tagged Sites
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Summary:SSRs is one of molecular markers technology based on DNA length polymorphism and an efficient tool for population genetic studies and primary genetic linkage maps construction. Because of a special primer marker, It's necessary to know a species DNA sequence in order to design primers for PCR testing. That is to say, there is a problem of SSR primer development. For the progress of SSR marker technology, the methods of developing SSR primer could be divided into four kinds: traditional constructing and screening genome library procedure, the SSR richment procedure, avoiding screening genome library procedure and database search procedure. This paper reviewed these four methods' operation processes and their advantages and disadvantages. In addition, transferability of SSR primers in closely related species were introduced in recent years.
ISSN:0253-9772