Effect of mycophenolate mofetil on the expression of early inflammatory reaction in diabetic rats

• Published in: Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban Vol. 33; no. 10; p. 913
• Main Authors: Peng, Lin, Zhang, Hao, Xu, Guo, Tang, Ren-hong
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2008
• Subjects: Animals; Chemokine CCL5 - biosynthesis; Chemokine CCL5 - genetics; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Experimental - metabolism; Diabetic Nephropathies - metabolism; Diabetic Nephropathies - prevention & control; Ectodysplasins - biosynthesis; Ectodysplasins - genetics; Inflammation - metabolism; Kidney - metabolism; Male; Mycophenolic Acid - analogs & derivatives; Mycophenolic Acid - therapeutic use; Random Allocation; Rats; Rats, Sprague-Dawley; RNA, Messenger - biosynthesis; RNA, Messenger - genetics
• ISSN: 1672-7347

To investigate the effect of mycophenolate mofetil(MMF) on early inflammatory reaction of renal lesion in streptozotocin(STZ)-induced diabetic rats. Thirty-six male Sprague-Dawley rats were randomly divided into 3 groups after uninephrectomy: normal control group, diabetic model group, and MMF-treated group. Six rats in each group were sacrificed at the 4th week and 14th week after STZ injection. Twenty-four hour urinary protein (24 h Upro) count was measured before death. The expressions of regulated on activation of normal T expressed and secreted (RANTES),ectodermal dysplasia (ED-1)and Col-IV protein in the renal tissue were detected by immunohistochemistry. The expression of RANTES mRNA in the renal tissue was detected by RT-PCR. MMF prevented the increasing of 24h Upro in diabetic rats,and the expressions of RANTES,ED-1,Col-IV protein and RANTES mRNA in the kidney of MMF-treated rats were significantly decreased. MMF plays an early renal protective role in diabetic nephropathy, possibly through inhibitio