Expression levels of CXCR4 on megakaryocytes and its ligand in bone marrow in children with acute idiopathic thrombocytopenic purpura

• Published in: Zhonghua er ke za zhi Vol. 42; no. 7; p. 499
• Main Authors: Sheng, Guang-yao, Huang, Xi-lian, Bai, Song-ting
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2004
• Subjects: Adolescent; Bone Marrow - metabolism; Chemokine CXCL12; Chemokines, CXC - blood; Child; Child, Preschool; Enzyme-Linked Immunosorbent Assay; Humans; Infant; Ligands; Megakaryocytes - metabolism; Purpura, Thrombocytopenic, Idiopathic - blood; Receptors, CXCR4 - biosynthesis
• ISSN: 0578-1310

Chemokine receptor CXCR4 and its ligand stromal-derived factor 1 alpha (SDF-1alpha) have been paid increasing attention for their involvement in megakaryocytic hematopoiesis. It has been revealed in recent years that they can induce mature and immature megakaryocytes (MKs) to migrate through bone marrow endothelial cells (BMEC) by increasing the affinity of MKs for BMEC. Thus MKs maturity and eventual release of platelet from MKs ensues. While maturity disturbance of MKs and impaired production of platelets have been regarded as the main pathogenesis of ITP, the mechanism of which still remains unclear. Therefore, a clear understanding of the levels of CXCR4 and SDF-1alpha within bone marrow in children with ITP will help us to elucidate further the mechanism of ITP as well as to provide direct theoretical evidence for predicting treatment effect and evaluating prognosis. Bone marrow were aspirated from 28 children with AITP and 12 normal children. Percoll density gradient and immunomagnetic beads method were