Biocomplex of subtilisin Carlsberg with chitosan as an effective biocatalyst for hydrolysis and synthesis of peptides

New biocatalysts, preparations of subtilisin Carlsberg immobilized on chitosan (a deacetylated derivative of chitin), were obtained. The enzyme content, hydrolytic activity, and ability to catalyze peptide bond formation in organic solvents were characterized for these preparations. The influence of...

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Published inBioorganicheskaia khimiia Vol. 34; no. 3; p. 371
Main Authors Bacheva, A B, Isakov, M S, Lysogorskaya, E N, Macquarrie, D J, Filippova, I Iu
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.05.2008
Subjects
Catalysis
Chitosan - chemistry
Hydrolysis
Oligopeptides - chemical synthesis
Oligopeptides - chemistry
Peptides - chemical synthesis
Peptides - chemistry
Subtilisins - chemistry
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Summary:New biocatalysts, preparations of subtilisin Carlsberg immobilized on chitosan (a deacetylated derivative of chitin), were obtained. The enzyme content, hydrolytic activity, and ability to catalyze peptide bond formation in organic solvents were characterized for these preparations. The influence of the form and composition of the biocomplex (content of the enzyme and glutaraldehyde, the cross-linking agent) and buffer pH on the biocata-lytic properties of the immobilized enzyme was studied in the reactions of peptide bond hydrolysis. The synthase activity of the preparations was investigated in the reaction of synthesis of Z-Ala-Ala-Leu-Phe-pNA in a 6 : 4 DMF-acetonitrile mixture in dependence on the reaction time. The yield of this product was 100% after only 40 min.
ISSN:0132-3423