The anti-fibrotic effects of Qidan granule in experimental silicosis
To investigate the anti-fibrotic effects of Qidan granule in rats. The rats were randomly divided into six experimental groups: normal group, model group, Qidan group, Tetrandrine group. All rats except normal group were treated with silicon dioxide (50 mg/rat) by intratracheal instillation to induc...
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Published in | Zhōnghuá yùfáng-yīxué zázhì Vol. 41; no. 4; p. 290 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | Chinese |
Published |
China
01.07.2007
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Subjects | |
Online Access | Get more information |
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Summary: | To investigate the anti-fibrotic effects of Qidan granule in rats.
The rats were randomly divided into six experimental groups: normal group, model group, Qidan group, Tetrandrine group. All rats except normal group were treated with silicon dioxide (50 mg/rat) by intratracheal instillation to induce silicosis. Qidan group and Tetrandrine group were treated with Qidan granule (3125 mg/kg) or treated with Tetrandrine (22 mg/kg) respectively. All the rats were sacrificed after 5 months. Calculate Lung/body coefficient by weighting the lung wet weight and the body weight of rats. Content of Hydroxyproline was measured by alkaline hydrolysis. The gene expression of transforming growth factor-beta1 was examined by using enzyme-linked immunosorbent assay (ELISA). Paraffin embedded lung sections with HE staining, VG staining and Gomori staining were observed under light microscope.
In Qidan group and Tetrandrine group, Lung/body coefficient and content of Hydroxyproline and expression of transforming growth factor-b |
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ISSN: | 0253-9624 |