The anti-fibrotic effects of Qidan granule in experimental silicosis

• Published in: Zhōnghuá yùfáng-yīxué zázhì Vol. 41; no. 4; p. 290
• Main Authors: Zhang, Hua-nan, Xin, Hong-tao, Zhang, Wei-dong, Jin, Chang-jun, Huang, Shan-ying, Zhang, Yan
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2007
• Subjects: Animals; Disease Models, Animal; Drugs, Chinese Herbal - pharmacology; Drugs, Chinese Herbal - therapeutic use; Phytotherapy; Pulmonary Fibrosis - pathology; Pulmonary Fibrosis - prevention & control; Rats; Rats, Wistar; Silicosis - drug therapy; Silicosis - metabolism; Silicosis - pathology; Transforming Growth Factor beta - biosynthesis
• ISSN: 0253-9624

To investigate the anti-fibrotic effects of Qidan granule in rats. The rats were randomly divided into six experimental groups: normal group, model group, Qidan group, Tetrandrine group. All rats except normal group were treated with silicon dioxide (50 mg/rat) by intratracheal instillation to induce silicosis. Qidan group and Tetrandrine group were treated with Qidan granule (3125 mg/kg) or treated with Tetrandrine (22 mg/kg) respectively. All the rats were sacrificed after 5 months. Calculate Lung/body coefficient by weighting the lung wet weight and the body weight of rats. Content of Hydroxyproline was measured by alkaline hydrolysis. The gene expression of transforming growth factor-beta1 was examined by using enzyme-linked immunosorbent assay (ELISA). Paraffin embedded lung sections with HE staining, VG staining and Gomori staining were observed under light microscope. In Qidan group and Tetrandrine group, Lung/body coefficient and content of Hydroxyproline and expression of transforming growth factor-b