Studies on the separation and purificaton of pumiloside from Nauclea officinalis by macroreticular resin

• Published in: Zhong yao cai Vol. 30; no. 10; p. 1301
• Main Authors: Xuan, Wei-dong, Bian, Jun, Chen, Hai-sheng
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2007
• Subjects: Adsorption; Alkaloids - chemistry; Alkaloids - isolation & purification; Camptothecin - analogs & derivatives; Camptothecin - chemistry; Camptothecin - isolation & purification; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal - chemistry; Drugs, Chinese Herbal - isolation & purification; Ethanol; Plants, Medicinal - chemistry; Reproducibility of Results; Resins, Synthetic - chemistry; Rubiaceae - chemistry; Technology, Pharmaceutical - methods; Water
• ISSN: 1001-4454

To investigate the technological parameters and the process for separation and purification of pumiloside (PML) from Nauclea officinalis Pierrc ex Pitard by macroreticular resin. PML was extracted by hot water, and the content of PML was determinated by HPLC method. The static capacity absorption, static elution ratios of five types of resin were studied respectively, and were compared to evaluate their absorption and desorption effect to PML. And then the absorption capacity, elution solution and elution volume of the AB-8 resin were researched to set up the optimum separation process for PML. Finally, PML was recrysallized from MeOH and identified by spectra analysis. The AB-8 resin had the best absorptive and separative properties to PML. The dynamic absorption ratio was 2.44 mg/g. The applicable process was as follows: the water extracted solution of Nauclea officinalis flow through the resin column repeatedly, after being eluted with 6BV of distilled water, the resin column was eluted with 4BV 30% ethano