Chloroplast transformation

In this chapter we briefly review the developmental history and current research status of chloroplast transformation and introduce the merits of chloroplast transformation as compared with the nuclear genome transformation. Furthermore, according to the chloroplast transformation achieved in oilsee...

Full description

Saved in:
Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 318; p. 285
Main Authors Lu, Xiao-Mei, Yin, Wei-Bo, Hu, Zan-Min
Format Journal Article
LanguageEnglish
Published United States 2006
Subjects
Biological Assay
Blotting, Southern
Brassica rapa - genetics
Cell Culture Techniques
Chloroplasts - genetics
Gene Expression
Gene Transfer Techniques
Genetic Vectors
Plant Leaves - genetics
Plants, Genetically Modified
Transformation, Genetic - genetics
Online AccessGet more information

Cover

More Information
Summary:In this chapter we briefly review the developmental history and current research status of chloroplast transformation and introduce the merits of chloroplast transformation as compared with the nuclear genome transformation. Furthermore, according to the chloroplast transformation achieved in oilseed rape (Brassica napus), we introduce the preparation of explants, transformation methods, system selection, identification methods of the transplastomic plants, and experimental results. The technical points, the bottleneck, and the further research directions of the chloroplast transformation are discussed in the notes.
ISSN:1064-3745