The plasma levels of transforming growth factor beta1 and the protein expressions of alpha-SMA, urokinase plasminogen activator and plasminogen activator inhibitor-1 in liver of patients with different grades of hepatic fibrosis

• Published in: Zhonghua gan zang bing za zhi Vol. 12; no. 7; p. 400
• Main Authors: Wu, Xi-Run, Lv, Min-He, Wang, Qi, Shi, Shui-Sheng, Guo, Wen-Dong
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2004
• Subjects: Actins - blood; Adult; Aged; Female; Hepatitis B, Chronic - blood; Hepatitis B, Chronic - complications; Humans; Liver Cirrhosis - blood; Liver Cirrhosis - etiology; Male; Middle Aged; Plasminogen Activator Inhibitor 1 - blood; Transforming Growth Factor beta - blood; Transforming Growth Factor beta1; Urokinase-Type Plasminogen Activator - blood
• ISSN: 1007-3418

To measure the plasma levels of transforming growth factor beta1 (TGFbeta1), the protein expression of alpha-SMA in hepatic stellate cells and urokinase plasminogen activator (uPA) and plasminogen activator inhibitor-1 (PAI-1), and study on the relationships between the plasma levels of TGFbeta1, the protein expression and the serum hyaluronic acid (HA) in patients with different grades of hepatic fibrosis. Thirty seven cases with hepatic fibrosis of different grades were classified according to HE and VG staining categories from 0 to 4, in which there were 8 cases in grade 1, 9 cases in grade 2, 7 cases in grade 3, 13 cases in grade 4. The plasma levels of TGFbeta1 and the serum levels of HA were detected by ELISA. The protein expressions of a-SMA, uPA and PAI-1 in fibrotic liver tissue were observed by immunohistochemistry. With the progression of hepatic fibrosis, the plasma levels of TGFbeta1 and the protein expression of a-SMA, uPA and PAI-1 in fibrotic liver tissue were increased. In grade 3 and 4, the