Effect of bone marrow mesenchymal stem cells on immunoregulation in H-2 haploidentical bone marrow transplantation mice

To explore immunoregulatory mechanism of mesenchymal stem cells (MSCs) in H-2 haploidentical bone marrow cells transplantation mice. BALB/c female mice irradiated with 8Gy 60Co gamma-rays were divided into two groups: MSCs group, infused cm-DiI labeled MSCs from female CB6F1 mice and monocytes from...

Full description

Saved in:
Bibliographic Details
Published inZhōnghuá xuèyèxué zázhì Vol. 28; no. 8; p. 505
Main Authors Hu, Kai-xun, Zhao, Shi-fu, Sun, Qi-yun, Guo, Mei, Ai, Hui-sheng
Format Journal Article
LanguageChinese
Published China 01.08.2007
Subjects
Animals
Bone Marrow Cells - immunology
Bone Marrow Transplantation - immunology
Female
Graft vs Host Disease - immunology
Male
Mesenchymal Stromal Cells - immunology
Mice
Mice, Inbred BALB C
Online AccessGet more information

Cover

More Information
Summary:To explore immunoregulatory mechanism of mesenchymal stem cells (MSCs) in H-2 haploidentical bone marrow cells transplantation mice. BALB/c female mice irradiated with 8Gy 60Co gamma-rays were divided into two groups: MSCs group, infused cm-DiI labeled MSCs from female CB6F1 mice and monocytes from the bone marrow and spleen of male CB6F1; Control group, only infused monocytes from the bone marrow and spleen of male CB6F1. T-lymphocyte subpopulation of peripheral blood cells, T and B cells proliferation stimulated by ConA and LPS, mixed lymphocyte reaction between donor and recipient and third part, the sry-gene chimerism of bone marrow, spleen and thymus of the recipient, the distribution of MSCs in the recipient, the incidence rate of GVHD and survival were observed. The CD3 at +90 d the percent of CD3+ CD4+ cells, and CD4/CD8 at +30 d in the MSCs group were higher than that in control post-transplantation, respectively (P < 0.05). The proliferation activity of B cells recovered more rapidly and that of T c
ISSN:0253-2727