Regulation mechanism of photopigments biosynthesis via light and oxygen in Rhodobacter azotoformans 134K20

• Published in: Wei sheng wu hsüeh pao Vol. 49; no. 3; p. 331
• Main Authors: Yue, Huiying, Huang, Xiao, Zhao, Chungui, Yang, Suping, Qu, Yinbo
• Format: Journal Article
• Language: Chinese
• Published: China 01.03.2009
• Subjects: Bacteriochlorophylls - metabolism; Carotenoids - metabolism; Chromatography, Thin Layer; Gene Expression Regulation, Bacterial - drug effects; Gene Expression Regulation, Bacterial - radiation effects; Light; Oxygen - pharmacology; Rhodobacter - drug effects; Rhodobacter - metabolism; Rhodobacter - radiation effects
• ISSN: 0001-6209

To provided a reliable and sensitive method and a series of specific absorption spectra data of different carotenoids and bacteriochlorophylla in anxoygenic phototrophic bacteria, and reveal mechanism of regulation of photosynthetic pigments by light and oxygen in Rhodobacter azotoformans 134K20. The metabolic diversity of photopigments regulated by oxygen and light was investigated by means of UV-VIS spectra and thin layer chromatography. The highest cell yield of strain 134K20 was obtained under aerobic conditions in the light. Nine types of photopigments including three yellow pigments, one red pigment, one purple pigment, two green pigments, and two blue pigments were synthesized, and yellow pigments synthetic genes were expressed on the higher level anaerobically in the light. Under aerobic conditions, the synthetic genes of two new red and one new purple pigment were triggered and expressed on the higher level, but the biosynthesis of yellow, blue and green pigments were inhibited by oxygen, another nin