An experimental study of cell apoptosis and correlative gene expression after tractive spinal cord injury in rats

• Published in: Chung-hua wai kʿo tsa chih Vol. 42; no. 23; p. 1434
• Main Authors: Liu, Lei, Shen, Bin, Yang, Jing, Lü, Bo, Yang, Xiao-ning, Zhou, Zong-ke, Pei, Fu-xing
• Format: Journal Article
• Language: Chinese
• Published: China 07.12.2004
• Subjects: Animals; Apoptosis - genetics; bcl-2-Associated X Protein; Disease Models, Animal; Female; Gene Expression; Genes, bcl-2 - genetics; Genes, p53 - genetics; Male; Proto-Oncogene Proteins c-bcl-2 - genetics; Rats; Rats, Sprague-Dawley; Spinal Cord Injuries - genetics; Spinal Cord Injuries - pathology
• ISSN: 0529-5815

To observe the cell apoptosis after tractive spinal cord injury in rats, determine expression of apoptosis correlative genes, and study the molecular mechanism of cell apoptosis. The T(13)-L(2) spinal cord of rats was injured by traction after the amplitude of P1-N1 wave decreased to 70% in postoperation than in preoperation through cortical somatosensory evoked potential (CSEP) monitor. Then rats were killed in 30 min, 6 h, 1, 4, 7, 14 and 21 d respectively after operation (n = 4). Cell apoptosis was examined by the flow cytometer and terminal deoxynucleotidyl transferase-mediated DUTP-biotin nick end labeling (TUNEL) reaction, the expression of p53, bax and bc1-2 genes was tested with immunohistochemistry. The flow cytometer test and TUNEL method showed that the apoptosis cell ratio raised in 6 h and reached at peak in 7 d after injury, and then declined till 21 d, they showed significant difference (P < 0.05, 0.01). TUNEL method showed that injured group had a large number of apoptosis glial cells in white