DNA fingerprinting analysis of silkworm embryo cell lines

DNA extraction and the polymerase chain reaction (PCR) were used on DNA genomes study of cell lines of Bombyx mori. DNA polymorphic marker analysis was conducted and DNA fingerprint of cell lines of Bombyx mori. was carried out using ISSR and RAPD. Primers that can reliably find polymorphic bands we...

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Bibliographic Details
Published inFen zi xi bao sheng wu xue bao Vol. 39; no. 6; p. 537
Main Authors Pan, Min Hui, Feng, Zhen Yue, Tian, Zhi Qiang, Liu, Min, Lu, Cheng
Format Journal Article
LanguageChinese
Published China 01.12.2006
Subjects
Animals
Bombyx - cytology
Bombyx - embryology
Bombyx - genetics
Cell Line
DNA Fingerprinting - methods
DNA Primers - genetics
Polymerase Chain Reaction
Random Amplified Polymorphic DNA Technique
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Summary:DNA extraction and the polymerase chain reaction (PCR) were used on DNA genomes study of cell lines of Bombyx mori. DNA polymorphic marker analysis was conducted and DNA fingerprint of cell lines of Bombyx mori. was carried out using ISSR and RAPD. Primers that can reliably find polymorphic bands were screened out. 26 ISSR primers were selected from them any available, and 797 polymorphic bands were abtained through PCR amplification in 9 samples, including 3 embryo cell lines of Bombyx mori (BmE-SWU1, BmE-SWU2, BmE-SWU3), 5 passage cell lines (BmE, BmN, Sf9, Sf21, Hi5) and the embryos from which BmE-SWU1 originated. The ration of polymorphic bands was 89.9%. 43 RAPD primers were selected out through PCR amplification, and 1205 polymorphic bands were obtained in 9 samples. The ration of polymorphic bands was 76.6%. There were many DNA polymorphic bands differences in the cell lines of Bombyx mori. The special DNA markers of the 3 embryo cell lines were found respectively. The similarity index Nei and genetic
ISSN:1673-520X